27-hydroxycholesterol 27ohc Search Results


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ELISA for estimation of Human 27-Hydroxycholesterol, 27-OHC in serum, plasma and other biological fluids
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Avanti Inc 27-hydroxycholesterol (27-ohc
A) Western-blot analysis of neuronal primary cultures treated with 100 nM rotenone for 18h. Trx80, Trx1 and Nrf2 band intensities are normalized against Actin (n=3 biological replicates). B) Western-blot analysis of neuronal primary cultures treated with 1 µM <t>27-hydroxycholesterol</t> for 2h. Trx80, Trx1 and Nrf2 are normalized against Actin (n=3 biological replicates). C) Western-blot quantification of Trx80 in 22-month old wild-type and Cyp27A1 overexpressing mice cortical homogenates (n=7 wild-type, n=7 Cyp27A1Tg). Results are shown as relative to controls. Statistical analysis was performed by Student t-test (*p<0.5; **p<0.01; ***p<0.001; **** p<0.0001).
27 Hydroxycholesterol (27 Ohc, supplied by Avanti Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/27-hydroxycholesterol+27ohc/bio_rxiv__2022__03__09__483648-137-5-7?v=Avanti+Inc
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27-hydroxycholesterol (27-ohc - by Bioz Stars, 2026-08
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A) Western-blot analysis of neuronal primary cultures treated with 100 nM rotenone for 18h. Trx80, Trx1 and Nrf2 band intensities are normalized against Actin (n=3 biological replicates). B) Western-blot analysis of neuronal primary cultures treated with 1 µM 27-hydroxycholesterol for 2h. Trx80, Trx1 and Nrf2 are normalized against Actin (n=3 biological replicates). C) Western-blot quantification of Trx80 in 22-month old wild-type and Cyp27A1 overexpressing mice cortical homogenates (n=7 wild-type, n=7 Cyp27A1Tg). Results are shown as relative to controls. Statistical analysis was performed by Student t-test (*p<0.5; **p<0.01; ***p<0.001; **** p<0.0001).

Journal: bioRxiv

Article Title: Neuron-derived Thioredoxin-80: a novel regulator of type-I interferon response in microglia

doi: 10.1101/2022.03.09.483648

Figure Lengend Snippet: A) Western-blot analysis of neuronal primary cultures treated with 100 nM rotenone for 18h. Trx80, Trx1 and Nrf2 band intensities are normalized against Actin (n=3 biological replicates). B) Western-blot analysis of neuronal primary cultures treated with 1 µM 27-hydroxycholesterol for 2h. Trx80, Trx1 and Nrf2 are normalized against Actin (n=3 biological replicates). C) Western-blot quantification of Trx80 in 22-month old wild-type and Cyp27A1 overexpressing mice cortical homogenates (n=7 wild-type, n=7 Cyp27A1Tg). Results are shown as relative to controls. Statistical analysis was performed by Student t-test (*p<0.5; **p<0.01; ***p<0.001; **** p<0.0001).

Article Snippet: Neurons were treated 1 μM 27-hydroxycholesterol (27-OHC, Avanti Instruchemie, The Netherlands) or vehicle (DMSO, Sigma) for 2h before collection.

Techniques: Western Blot